In vitro effect of rifampicin and its derivatives on energy transfer reactions in mitochondria.
نویسندگان
چکیده
In order to clarify the biological effects of rifampicin and its derivatives on liver cells, the actions of these antibiotics on the energy transfer reactions were observed in rat liver mitochondria. Rifampicin, 25-desacetyl rifampicin and rifampicin quinone at concentrations of 2.8x10-5-2.7x10-4M had little effect on the respiration and several energy transfer reactions in rat liver mitochondria, while similar concentrations of 3-formyl rifamycin SV caused a marked decrease in respiratory control and ADP : O ratio with enhancing state 4 respiration, activated Mg++ -dependent ATPasc and also inhibited ATP-32Pi exchange reaction. These results suggest that 3-formyl rifam ycin SV, though not rifampicin itself, acts as a true uncoupler in vitro on rat liver mitochondria and its uncoupling action is fairly powerful.
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عنوان ژورنال:
- The Journal of antibiotics
دوره 27 3 شماره
صفحات -
تاریخ انتشار 1974